qRT-PCR analysis of the impact of the various stresses on transcription of virulence-associated genes of C. jejuni . Total RNA was isolated, and the expression of ciaB, dnaJ and htrA was measured immediately after exposure to each stress. All data were normalized to the level of expression of the 16S rRNA gene and are presented relatively to the non-stress control. Therefore, the non-stressed condition has a fold value of 1. Data are representative of three independent experiments from three RNA extracts.
Bui et al. BMC Microbiology 2012 12:232 doi:10.1186/1471-2180-12-232