SspA is required for cell adherence and A/E lesion formation. HEp-2 cells were infected by wild type EHEC EDL933 (A) and its mutant derivatives of sspA (B), sspA pQEsspA (C), sspA pQEsspA84-86 (D), hns (E) and hns sspA (F). Bacterial adherence was examined by phase-contrast images (left panels) and the actin cytoskeleton of infected HEp-2 cells by fluorescent microscopic images (right panels). Representative images are shown. Black and white arrowheads indicate bacteria and A/E lesions, respectively.
Hansen and Jin BMC Microbiology 2012 12:231 doi:10.1186/1471-2180-12-231