SspA is upstream of H-NS in the regulatory network of virulence gene expression in EHEC. The expression of virulence genes in wild type EHEC EDL933 (lane 1), sspA (lane 2), hns (lane 3) and hns sspA (lane 4) mutant derivatives was determined by primer extension analyses using labeled DNA oligos specific to the transcripts of LEE1/ler (A), LEE2/espZ (B), LEE3/mpc (C), LEE4/sepL (D), LEE5/tir (E), map (F), grlRA (G) and stcE (H). In each reaction, the ompA transcript served as an internal control. Samples were prepared and analyzed as described in the legend of Figure 1. The relative transcript levels of target genes normalized to that of ompA are indicated by the numbers in parenthesis.
Hansen and Jin BMC Microbiology 2012 12:231 doi:10.1186/1471-2180-12-231