|Differential Immunity profiles of each LES phage in PAO1|
|Efficiency of plating values||φ2||φ3||φ4|
|PAO1 naive host||1.0||1.0||1.0|
|Single φ2 lysogen||< 1x10 -9||< 1x10 -9||0.017|
|Single φ3 lysogen||0.91||< 1x10 -9||0.37|
|Single φ4 lysogen||1.09||0.94||3.3x10 -9|
Immunity profiles of each LES phage were determined by plaque assay. Phage dilution series were spotted onto non-Lysogenic PAO1 and PLPL lawns. Efficiency of plating = the ratio of plaques observed (at the appropriate phage dilution) on the most permissive host (non-lysogenic PAO1)/plaques observed on assay host (PLPL harbouring LESφ2, LESφ3 or LESφ4 prophages). If no plaques were observed when neat phage suspensions of 1010 p.f.u ml-1 were used, an eop value < 1x10-9 was recorded.
James et al.
James et al. BMC Microbiology 2012 12:216 doi:10.1186/1471-2180-12-216