Table 1

Differential Immunity profiles of each LES phage in PAO1
Efficiency of plating values φ2 φ3 φ4
PAO1 naive host 1.0 1.0 1.0
Single φ2 lysogen < 1x10 -9 < 1x10 -9 0.017
Single φ3 lysogen 0.91 < 1x10 -9 0.37
Single φ4 lysogen 1.09 0.94 3.3x10 -9

Immunity profiles of each LES phage were determined by plaque assay. Phage dilution series were spotted onto non-Lysogenic PAO1 and PLPL lawns. Efficiency of plating = the ratio of plaques observed (at the appropriate phage dilution) on the most permissive host (non-lysogenic PAO1)/plaques observed on assay host (PLPL harbouring LESφ2, LESφ3 or LESφ4 prophages). If no plaques were observed when neat phage suspensions of 1010 p.f.u ml-1 were used, an eop value < 1x10-9 was recorded.

James et al.

James et al. BMC Microbiology 2012 12:216   doi:10.1186/1471-2180-12-216

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