PCR confirmation of all PAO1 LES phage lysogens. Lysogens were isolated from turbid plaques following sequential infection of PAO1 with pure stocks of each LES phage. Lysogens were considered resistant if no plaques were observed following exposure to increasingly high titre phage suspensions (up to MOI 100). The presence of each prophage was confirmed using multiplex PCR with specific primer sets for each LES phage yielding differentially sized products: 325 bp (LESφ3); 250 bp (LESφ2); 100 bp (LES φ 4).
James et al. BMC Microbiology 2012 12:216 doi:10.1186/1471-2180-12-216