Open Access Research article

Role of trehalose in heat and desiccation tolerance in the soil bacterium Rhizobium etli

Mercedes Reina-Bueno1, Montserrat Argandoña1, Joaquín J Nieto1, Alba Hidalgo-García3, Fernando Iglesias-Guerra2, María J Delgado3 and Carmen Vargas1*

  • * Corresponding author: Carmen Vargas cvargas@us.es

  • † Equal contributors

Author Affiliations

1 Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Profesor García González 2, Seville, 41012, Spain

2 Department of Organic and Pharmaceutical Chemistry, Faculty of Pharmacy, University of Seville, Profesor García González, Seville, 41012, Spain

3 Estación Experimental del Zaidín, CSIC, PO Box 419, Granada, 18080, Spain

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BMC Microbiology 2012, 12:207  doi:10.1186/1471-2180-12-207

Published: 17 September 2012

Additional files

Additional file 1:

Table S1. R. etli genes involved in trehalose and glutamate metabolis.

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Additional file 2:

Figure S1. Genomic analysis of R. etli pathways involved in trehalose metabolism. (A) Genomic context of genes involved in trehalose metabolism. Position and clustering of genes included in 1 are indicated. (B) Neighbor-joining tree based on proteins belonging to families 13 and 15 of glycosydases, including the three TreC-like proteins from R. etli. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The E. coli and Rhrodothermus marinus representatives were used as outgroup. The evolutionary distances were computed using the Poisson correction method and are in the units of the number of amino acid substitutions per site. The rate variation among sites was modeled with a gamma distribution (shape parameter = 1). All positions containing gaps and missing data were eliminated from the dataset (complete deletion option). Bootstrap probabilities (as percentage) were determined from 1000 resamplings.

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Additional file 3:

Figure S2. Growth of R. wild type (WT) and the otsAch mutant CMS310 with trehalose and glucose as the sole carbon source. Cells were grown in at 28°C in B- minimal medium with 20 mM trehalose or glucose and 0.0 or 0.2 M NaCl.

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