Carotenoid biosynthesis and overproduction in Corynebacterium glutamicum
Chair of Genetics of Prokaryotes, Faculty of Biology & CeBiTec, University of Bielefeld, P.O. Box 100131, D-33501, Bielefeld, Germany
BMC Microbiology 2012, 12:198 doi:10.1186/1471-2180-12-198Published: 10 September 2012
Additional file 1:
Table S1. Comparison of the crt genes from different corynebacteria, for which genome sequence information is available in the database (NCBI). The rows show the gene identifier (top) and accession number (middle) for each crt gene of the corresponding species and the amino acid identity to the respective crt gene product from C. glutamicum ATCC 13032 (bottom).
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Additional file 2:
Figure S1. Phylogenetic tree of phytoene desaturase from different corynebacteria. Numbers at the nodes represent bootstrap values. Gene identifiers are given in Additional file 1: Table S2.
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Additional file 3:
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Additional file 4:
Figure S2. HPLC chromatograms of carotenoids extracted from C. glutamicum ΔcrtB strains (A) and ΔcrtI (B).Detection by absorption at 470 nm. (A) Elution profiles of carotenoids extracted from C. glutamicum WT (blue), ΔcrtB(pEKEx3) (red), ΔcrtB(pEKEx3-crtB) (green), ΔcrtB(pEKEx3-crtB2) (pink). (B) Elution profiles of carotenoids extracted from C. glutamicum WT (blue), ΔcrtI(pEKEx3) (red), ΔcrtI(pEKEx3-crtI) (green), ΔcrtI(pEKEx3-crtI2-1/2) (pink).
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Additional file 5:
Figure S3. Absorption spectra of cell extracts of C. glutamicum WT and crt deletion strains. The extract of the strains C. glutamicum ΔcrtEb (black line) and ΔcrtY (grey line) show an additional absorption maximum at about 500 nm compared to the wild type (red line). C. glutamicum ΔcrtB (dotted line) and ΔcrtI (dashed line) show no absorption.
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Additional file 6:
Figure S4. HPLC elution profiles of carotenoids extracted from C. glutamicum ΔΔ strains. Detection by absorption at 470 nm. (A) Elution profiles of carotenoids extracted from C. glutamicum ΔΔ(pEKEx3/pVWEx1) (blue), ΔΔ(pEKEx3-crtB/pVWEx1-crtI) (red), ΔΔ(pEKEx3-crtB2/pVWEx1-crtI) (green). The red and green chromatograms show the accumulation of carotenoids including lycopene (L) which elutes after about 18 min. (B) Elution profiles of carotenoids extracted from C. glutamicum ΔΔ(pEKEx3/pVWEx1) (blue) and ΔΔ(pEKEx3-crtI2-1/2/pVWEx1-crtB2) (red).
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