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Pyrosequencing-based analysis reveals a novel capsular gene cluster in a KPC-producing Klebsiella pneumoniae clinical isolate identified in Brazil

Pablo Ivan Pereira Ramos1, Renata Cristina Picão24, Eliana Carolina Vespero3, Marsileni Pelisson3, Luiz Fernando Goda Zuleta1, Luiz Gonzaga P Almeida1, Alexandra L Gerber1, Ana Tereza R Vasconcelos1, Ana Cristina Gales4 and Marisa Fabiana Nicolás1*

Author Affiliations

1 Laboratório Nacional de Computação Científica (LNCC), Petrópolis, Rio de Janeiro, Brazil

2 Instituto de Microbiologia Paulo de Góes, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil

3 Departamento de Patologia Clínica, Análises Clínicas e Toxicologia, Universidade Estadual de Londrina, Londrina, Brazil

4 Laboratório ALERTA, Divisão de Doenças Infecciosas, Universidade Federal de São Paulo, São Paulo, Brazil

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BMC Microbiology 2012, 12:173  doi:10.1186/1471-2180-12-173

Published: 11 August 2012

Additional files

Additional file 1:

Cluster analysis of 103 RFLP patterns after MST analysis. MST distances between serotypes are represented as alignment scores, with 0.75 used as the scale-adjusted threshold for distinguishing two serotypes. K. pneumoniae Kp13 is labeled as KP13, while the other serotypes follow the C-pattern nomenclature from Brisse et al. [29].

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