Figure 2.

Predicted protein sequence of a P. aeruginosa AES-1R hypothetical protein ((A); AES_7139; spot 43a-e) characterized by MALDI-MS and LC-MS/MS (B). Peptide Mass, peptides observed by either LC-MS/MS (charge states > +1) or by peptide mapping (charge state +1); Sequence, peptide sequence defined by assignment of y- and b-ions and confirmed by manual interpretation of the MS/MS spectra (920.4535 (+1); matched by mass alone); Note, modifications to sequence identified by MS. Peptides identified by MS are underlined in the protein sequence. Note the non-tryptic N-terminal peptide (958.5200 (+3) m/z), suggesting the methionine at position 7 is the true N-terminus. Cysteine residue potentially involved in disulfide bond/homodimer formation is marked with (*).

Hare et al. BMC Microbiology 2012 12:16   doi:10.1186/1471-2180-12-16
Download authors' original image