Table 3

Effects of bacterial probiotic supplementation on rumen fermentation characteristics during acidosis induced by feed challenges
Treatments1 Pvalue (Probvs.C)2
C (n = 4) P(n = 4) Lp + P(n = 4) Lr + P(n = 4) SEM P Lp + P Lr + P
Wheat-induced lactic acidosis
Ruminal pH
Mean 5.25 4.55 4.76 4.33 0.15 0.001 0.02 0.0001
Minimum 4.87 4.28 4.45 4.17 0.19 0.03 0.12 0.01
Total VFAs, mM 93.6 33.9 76.7 33.5 14.4 0.01 0.32 0.001
Acetate3, mol % 72.6 87.0 78.1 92.5 4.10 0.01 0.34 0.001
Propionate, mol % 12.2 6.63 10.6 3.82 2.49 0.10 0.63 0.02
Butyrate, mol % 12.8 5.79 10.2 3.52 1.94 0.01 0.33 0.001
Minor VFAs4, mol % 2.33 0.56 1.11 0.14 0.40 0.001 0.02 0.0001
Lactate, mM 33.8 71.1 64.9 79.6 9.28 0.005 0.02 0.001
NH3-N, mM 6.53 3.58 4.25 2.44 1.16 0.03 0.09 0.003
Ethanol, mM 6.57 12.4 17.2 14.4 1.85 0.02 0.0001 0.003
Corn-induced butyric subacute acidosis
Ruminal pH
Mean 5.49 5.61 5.74 5.65 0.08 0.30 0.03 0.18
Minimum 5.17 5.28 5.63 5.46 0.12 0.50 0.01 0.09
Total VFAs, mM 107 85.7 81.6 94.4 7.79 0.03 0.01 0.19
Acetate, mol % 63.2 67.4 68.7 66.9 1.75 0.08 0.03 0.13
Propionate, mol % 17.0 14.2 14.5 15.5 1.09 0.07 0.19 0.31
Butyrate, mol % 16.9 14.7 12.1 13.5 1.41 0.26 0.02 0.09
Minor VFAs, mol % 2.88 3.68 4.29 4.09 0.44 0.20 0.02 0.05
Lactate, mM 3.40 3.78 3.22 3.49 0.86 0.71 0.87 0.92
NH3-N, mM 0.74 0.73 0.71 1.15 0.98 0.99 0.98 0.76
Ethanol, mM 3.15 3.60 2.72 2.74 0.36 0.38 0.40 0.42
Beet pulp-induced propionic subacute acidosis
Ruminal pH
Mean 5.67 5.94 5.87 5.93 0.08 0.02 0.08 0.02
Minimum 5.55 5.84 5.72 5.83 0.11 0.05 0.27 0.06
Total VFAs, mM 114 112 104 100 6.66 0.89 0.33 0.16
Acetate, mol % 67.4 68.6 68.4 67.8 1.15 0.46 0.55 0.79
Propionate, mol % 22.5 21.5 21.9 22.3 0.83 0.38 0.61 0.88
Butyrate, mol % 8.52 8.40 8.18 8.34 0.49 0.86 0.85 0.77
Minor VFAs, mol % 1.50 1.48 1.52 1.46 0.26 0.94 0.96 0.91
Lactate, mM 2.71 2.01 1.52 2.01 1.46 0.73 0.56 0.73
NH3-N, mM 0.55 0.51 0.57 0.57 0.74 0.97 0.99 0.98
Ethanol, mM 3.34 3.22 2.64 2.84 0.48 0.86 0.31 0.47

1 Treatment with C = control without probiotic; P = Propionibacterium P63; Lp + P = L. plantarum + P63; Lr + P = L. rhamnosus + P63. 2 Effect of each probiotic treatment vs. control wether (C). 3 Individual VFAs are expressed in % of total VFAs. 4 Minor VFAs: sum of iso-butyrate, iso-valerate, valerate and caproate. The fermentation characteristics were determined on d3 at 6 h after feed challenges induced acidosis.

Lettat et al.

Lettat et al. BMC Microbiology 2012 12:142   doi:10.1186/1471-2180-12-142

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