2-DE analysis and Western blot for identification of immunogens from filtrate proteins of A. fumigatus. (A) 2-DE of filtrate proteins of A. fumigatus during growth in YEPG medum at 37°C for 14 days. (B) Immunoblot using pooled sera from proven IA patients. Filtrate proteins (150 μg) were separated by isoelectric focusing on Immobiline Dry strips (pH range, 3-10) followed by SDS-PAGE and silver staining. Standard molecular mass markers are indicated. Distinct protein spots (n = 39) with specific IgG immunoreactivity, as seen in corresponding immunoblots (B), were subjected to tryptic digestion followed by MALDI-TOF-MS analysis for identification (marked with arrow). The 17 proteins identified are numbered and listed in Table 2. Spot No. 2A-2 M was identified as thioredoxin reductase GliT.
Shi et al. BMC Microbiology 2012 12:11 doi:10.1186/1471-2180-12-11