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Open Access Research article

Proteins other than the locus of enterocyte effacement-encoded proteins contribute to Escherichia coli O157:H7 adherence to bovine rectoanal junction stratified squamous epithelial cells

Indira T Kudva1*, Robert W Griffin2, Bryan Krastins36, David A Sarracino36, Stephen B Calderwood245 and Manohar John247

Author Affiliations

1 Food Safety and Enteric Pathogens Research Unit, National Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Ames, Iowa, 50010, USA

2 Division of Infectious Diseases, Massachusetts General Hospital, Boston, Massachusetts, 02114, USA

3 Harvard Partners Center for Genetics and Genomics, 65 Landsdowne Street, Cambridge, Massachusetts, 02139, USA

4 Department of Medicine, Harvard Medical School, Boston, Massachusetts, 02114, USA

5 Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts, 02114, USA

6 Present Address: Thermo-Fisher Scientific, Cambridge, Massachusetts, 02139, USA

7 Present Address: Pathovacs Inc., Ames, Iowa, 50010, USA

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BMC Microbiology 2012, 12:103  doi:10.1186/1471-2180-12-103

Published: 12 June 2012

Abstract

Background

In this study, we present evidence that proteins encoded by the Locus of Enterocyte Effacement (LEE), considered critical for Escherichia coli O157 (O157) adherence to follicle-associated epithelial (FAE) cells at the bovine recto-anal junction (RAJ), do not appear to contribute to O157 adherence to squamous epithelial (RSE) cells also constituting this primary site of O157 colonization in cattle.

Results

Antisera targeting intimin-γ, the primary O157 adhesin, and other essential LEE proteins failed to block O157 adherence to RSE cells, when this pathogen was grown in DMEM, a culture medium that enhances expression of LEE proteins. In addition, RSE adherence of a DMEM-grown-O157 mutant lacking the intimin protein was comparable to that seen with its wild-type parent O157 strain grown in the same media. These adherence patterns were in complete contrast to that observed with HEp-2 cells (the adherence to which is mediated by intimin-γ), assayed under same conditions. This suggested that proteins other than intimin-γ that contribute to adherence to RSE cells are expressed by this pathogen during growth in DMEM. To identify such proteins, we defined the proteome of DMEM-grown-O157 (DMEM-proteome). GeLC-MS/MS revealed that the O157 DMEM-proteome comprised 684 proteins including several components of the cattle and human O157 immunome, orthologs of adhesins, hypothetical secreted and outer membrane proteins, in addition to the known virulence and LEE proteins. Bioinformatics-based analysis of the components of the O157 DMEM proteome revealed several new O157-specific proteins with adhesin potential.

Conclusion

Proteins other than LEE and intimin-γ proteins are involved in O157 adherence to RSE cells at the bovine RAJ. Such proteins, with adhesin potential, are expressed by this human pathogen during growth in DMEM. Ongoing experiments to evaluate their role in RSE adherence should provide both valuable insights into the O157-RSE interactions and new targets for more efficacious anti-adhesion O157 vaccines.

Keywords:
O157; Rectoanal junction; LEE; Adherence; DMEM; GeLC-MS/MS