Table 5

Bacterial strains and plasmids used in this study

Strain or plasmid

Relevant characteristicsa

Reference or source


Escherichia coli

DH5α

recA lacZΔM15

[27]

CECT831

Indicator strain of mangotoxin production

CECTb

Pseudomonas fluorescens

Pf-5

Complete genome sequenced and free access.

[28]

Pseudomonas syringae pv. syringae

B728a

Complete genome sequenced and free access

[16]

UMAF0158

Wild type isolated from mango, mangotoxin producer, Nfr

[12]

UMAF0158-2βB7

UMAF0158-3αE10

miniTn5 mutants of UMAF0158 in gacA and gacS respectively, defective in mangotoxin, Kmr, Nfr

[15]

UMAF0158-3νH1

UMAF0158-6νF6

miniTn5 mutants of UMAF0158, defective in mangotoxin production, Kmr, Nfr

[15]

UMAF2-6-3H1

UMAF2-6A

miniTn5 mutants from UMAF0158 and complemented with plasmid pCG2-6, production of mangotoxin restored, Kmr, Ampr, Nfr

This study

[15]

UMAF0158::ORF0c

ORF0 mutant of UMAF0158, ORF0::pCR-ORF0, Kmr, Nfr

This study

UMAF0158::ORF1

ORF1 mutant of UMAF0158, ORF1::pCR::ORF1, Kmr, Nfr

This study

UMAF0158::ORF2

ORF2 mutant of UMAF0158, ORF2::pCR::ORF2, Kmr, Nfr

This study

UMAF0158::mgoB

mgoB (ORF3) mutant of UMAF0158, mgoB::pCR::mgoB; Kmr, Nfr

This study

UMAF0158::mgoC

mgoC (ORF4) mutant of UMAF0158, mgoC::pCR::mgoC; Kmr, Nfr

This study

UMAF0158::mgoA

mgoA (ORF5) mutant of UMAF0158, mgoA::pCR::mgoA; Kmr, Nfr

This study

UMAF0158::mgoD

mgoD (ORF6) mutant of UMAF0158, mgoD::pCR::mgoD; Kmr, Nfr

This study

UMAF0158ΔmgoA

mgoA (ORF5) mutant of UMAF0158 by deletion, Nfr

This study

Plasmids

pGEM-T

Cloning vector, Ampr

Invitrogen, California, USA

pCR2.1

Cloning vector lacZ, Kmr, Ampr,

Invitrogen, California, USA

pBBR1MCS-5

Cloning vector, Gmr

[29]

pMP220

Promoter-probe vector containing a promoterless lacZ gene

[30]

pCR::ORF0

integrative plasmid pCR2.1 carrying ORF0

This study

pCR::ORF1

integrative plasmid pCR2.1 carrying ORF1

This study

pCR::ORF2

integrative plasmid pCR2.1 carrying ORF2

This study

pCR::mgoB

integrative plasmid pCR2.1 carrying mgoB

This study

pCR::mgoC

integrative plasmid pCR2.1 carrying mgoC

This study

pCR::mgoA

integrative plasmid pCR2.1 carrying mgoA

This study

pCR::mgoD

integrative plasmid pCR2.1 carrying mgoD

This study

pCG2-6

genomic clone of UMAF0158 GenBank-DQ532441

[15]

pLac36

From mgoB to mgoD cloned in pBBR1MCS-5

This study

pLac56

mgoA and mgoD cloned in pBBR1MCS-5

This study

pLac6

mgoD cloned in pBBR1MCS-5

This study

pMPmgo

pMP220 vector containing the putative promoters of mgo operon

This study

pEMG

integrative plasmid for deletion mutagenesis, Kmr.

[31]

pSW-2

plasmid carrying I-SceI gene for deletion mutagenesis, Gmr.

[31]


a) Ampr: ampicillin resistance; Gmr: gentamycin resistance; Kmr: kanamycin resistance; Nfr: Nitrofurantoin resistance. b) CECT: Spanish Type Culture Collection. c) ORF0 was named in this way because it was cloned as an uncompleted ORF

Arrebola et al. BMC Microbiology 2012 12:10   doi:10.1186/1471-2180-12-10

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