Table 3

Analysis of mangotoxin production using miniTn5 and insertional mutants obtained from Pseudomonas syringae pv.syringae UMAF0158, before and after transformation with plasmids containing total or partial mgo operon involved in mangotoxin production

Mangotoxin production in strains containing complementing plasmidsa


Strain

ORF mutated

None

pCG2-6

pLac36

pLac56

pLac6


UMAF0158

nab

+

na

na

na

na

UMAF0158::mgoB

mgoB

+

+

+

+

+

UMAF0158-3νH1

mgoC

-

+

+

-

-

UMAF0158::mgoC

mgoC

-

+

+

-

-

UMAF0158-6νF6

mgoA

-

+

+

+

-

UMAF0158::mgoA

mgoA

-

+

+

+

-

UMAF0158::mgoD

mgoD

-

+

+

+

+


a) +: presence of inhibition halo; -: absence of inhibition halo

b) na, not applicable

pCG2-6: genomic clone of UMAF0158. GenBank access DQ532441 (Table 4)

pLac36: mgoB, mgoC, mgoA and mgoD cloned in pBBR1MCS-5 (Table 4)

pLac56: mgoA and mgoD cloned in pBBR1MCS-5 (Table 4)

pLac6: mgoD cloned in pBBR1MCS-5 (Table 4)

Arrebola et al. BMC Microbiology 2012 12:10   doi:10.1186/1471-2180-12-10

Open Data