Cell wall integrity assays with H99 C. neoformans cells left untreated (H99) or exposed to FLC (H99F) at a sub-MIC concentration of 10 mg/l for 90 min at 30°C. Cells were grown at the same temperature for 48 h on YEPD supplemented with calcofluor white (CFW), Congo red, sodium dodecyl sulphate (SDS) and caffeine. Aliquots of cells were applied onto the agar surface with 10-fold serial dilutions.
Florio et al. BMC Microbiology 2011 11:97 doi:10.1186/1471-2180-11-97