Co-immunoprecipitation of FlbD and the FliX mutants. Extracts of JG1172 cells expressing various fliX alleles were incubated with agarose beads coated with anti-FlbD antibodies. Proteins bound to the bead complexes were detected using anti-FliX antibodies following SDS-PAGE electrophoresis. The immunoblot was developed to an extended period of time to visualize the band of FliXΔ117-118 (indicated by the arrow).
Xu et al. BMC Microbiology 2011 11:89 doi:10.1186/1471-2180-11-89