Table 2

Specific nucleotide positions for identification of pathogenic species within the section Fumigati (inside parentheses the number of sequences studied for each species).

Species

β-tubulin sequence

Rodlet A sequence


Aspergillus fumigatus

T24 # (96)

Polymorphism not found (47)


Aspergillus fumigatiaffinis

DelG93 # (6)

Polymorphism not found (3)


Aspergillus lentulus *

T58A and C99 (48)

Polymorphism not found (39)


Aspergillus viridinutans

Polymorphism not found (20)

A32G or C33T (2)


Neosartorya fennelliae

InsA87 # or A105G # (18)

NI


Neosartorya fischeri

DelC99 or A131T (5)

NI


Neosartorya hiratsukae

G53 and G113A (10)

C55T or G62C or T76C or C82A (6)


Neosartorya pseudofischeri

G116C (15)

Polymorphism not found (5)


Neosartorya udagawae

A114G (22)

A56G or C82T (16)


* Aspergillus fumisynnematus may also present these β-tubulin polymorphisms but very few sequences are still available.

# Nomenclature: T24 - a thymine is present in position 24; DelG93 - deletion of the guanine in position 93; InsA87 - insertion of an adenine in position 87; A105G - replacement of an adenine by a guanine in position 105. The position numbers result from the gene alignment (Figures 2 and 3) and position 1 is located in the beginning of forward primer. (NI - not enought information, only one sequence was available).

Serrano et al. BMC Microbiology 2011 11:82   doi:10.1186/1471-2180-11-82

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