Microscopy of cells in the stationary phase microtiter biofilm model. The images (40 × magnification) show the attachment of pneumococci to the surface of microtiter plates after 24 hours of incubation. The wt strain TIGR4 (panel A), the comD mutant (panel B), the comC mutant (panel C) and the comC mutant with the addition of CSP2 (panel D) were compared. Biofilm images are taken on crystal violet stained cells observed in bright filed using the 40 × objective of a Leica DM1000 Microscope and a DFC digital camera.
Trappetti et al. BMC Microbiology 2011 11:75 doi:10.1186/1471-2180-11-75