Additional file 3.
Characterization of E. faecalis ΔbgsB cell walls. A Thin-layer chromatography of cell membrane total lipid extracts of E. faecalis 12030 wild type (lane 1 and 4), 12030ΔbgsB (lane 2 and 5), 12030ΔbgsA (lane 3 and 6). TLC plates were developed using a solvent system of CHCl3/MeOH/H20 (65:25:4, v/v/v). Staining lane 1 - 3 molybdenum blue, lane 4 - 6 ninhydrin. B SDS PAGE of bacterial whole protein extracts. The material was extracted by disrupting the cells with glass-beads, boiling in Laemmli buffer, separated by 4-12% Bis-Tris gels and stained with Coomassie blue.
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Theilacker et al. BMC Microbiology 2011 11:67 doi:10.1186/1471-2180-11-67