Open Access Highly Accessed Research article

Lateral gene transfer of streptococcal ICE element RD2 (region of difference 2) encoding secreted proteins

Izabela Sitkiewicz12*, Nicole M Green1, Nina Guo1, Laurent Mereghetti13 and James M Musser1

Author Affiliations

1 Center for Molecular and Translational Human Infectious Diseases Research, The Methodist Hospital Research Institute, and Department of Pathology, The Methodist Hospital, Houston, Texas, 77030, USA

2 Department of Epidemiology and Clinical Microbiology, National Medicines Institute. Warszawa, Poland

3 Université François-Rabelais, Faculté de Médecine, EA 3854 "Bactéries et Risque Materno-Foetal", Tours, France

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BMC Microbiology 2011, 11:65  doi:10.1186/1471-2180-11-65

Published: 1 April 2011

Additional files

Additional file 1:

Table S1: Streptococcal strains used in the study

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Additional file 2:

Table S2: Primers used for the mutant construction

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Additional file 3:

Supplemental Methods

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Additional file 4:

Figure S1: Conformation of proper mutant construction

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Figure S2: Determination of MIC values for mitomycin C and hydrogen peroxide

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Additional file 6:

Table S3: Homologs of RD2 genes found in GBS

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Additional file 7:

Figure S3: Induction of prophages and ICE elements in MGAS6180 after treatment with mitomycin C and hydrogen peroxide.

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