Comparison of the WT and the arcA mutant for surface appendages and flagella via microscopy. Scanning electron microscopy (SEM) was used to evaluate the WT (A) and the arcA mutant (C) for the presence/absence of surface appendages and negative staining followed by transmission electron microscopy (TEM) was used to evaluate the WT (B) and the arcA mutant (D) for the presence/absence of flagella. Cells were grown anaerobically in LB-MOPS-X media and the samples were prepared as described in Materials and Methods.
Evans et al. BMC Microbiology 2011 11:58 doi:10.1186/1471-2180-11-58