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Analysis of the ArcA regulon in anaerobically grown Salmonella enterica sv. Typhimurium

Matthew R Evans14, Ryan C Fink15, Andres Vazquez-Torres2, Steffen Porwollik3, Jessica Jones-Carson2, Michael McClelland3 and Hosni M Hassan1*

Author Affiliations

1 Department of Microbiology, North Carolina State University, Raleigh, North Carolina 27695-7615 USA

2 Department of Microbiology, University of Colorado School of Medicine, Denver, Colorado 80262 USA

3 The Vaccine Research Institute of San Diego, 10835 Road to the Cure, Suite 105, San Diego, California 92121 USA

4 Pfizer, Inc., 4300 Oak Park Road, Sanford, NC 27330-9550 USA

5 Department of Food Science and Nutrition, University of Minnesota, St. Paul, MN 55108-1038 USA

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BMC Microbiology 2011, 11:58  doi:10.1186/1471-2180-11-58

Published: 21 March 2011

Additional files

Additional file 1:

Analysis of the ArcA regulon in anaerobically grown Salmonella enterica sv. Typhimurium. Identification of ArcA by Western blot; Effects of H2O2 on viability of the ArcA mutant; List of genes differentially regulated by ArcA; and List of genes shared with the Fnr regulon. A. Supplemental Methods: Western blot analysis of ArcA. H2O2 survival assays. B. Supplemental Figures:

    Figure S1
. Western blot of total proteins of the WT, arcA mutant, and arcA-/parcA complement strains.
    Figure S2
. Effects of hydrogen peroxide on viability of the WT and the arcA mutant under anerobiosis. C. Supplemental Tables: Table S1. Differentially expressed genes and the presence/absence of putative ArcA-binding motifs in their 5' regions. Table S2. Comparison of the 120 genes shared between the ArcA and the Fnr regulons of S. Typhimurium under anaerobiosis.

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