Figure 4.

Localization of PnxIIIA and the protein interaction analysis of rPnxIIIA. (A) Western blotting analysis of the cell fraction prepared from P. pneumotropica ATCC 35149 cells and culture by using anti-rPnxIIIA IgG. Lanes: 1, SC fraction; 2, IM fraction; 3, OM fraction; 4, UC fraction. (B) Western blotting analysis of the cell fraction prepared from P. pneumotropica ATCC 35149 cells and culture by using anti-rPnxIIIE IgG. Lanes: 1, SC fraction; 2, IM fraction; 3, OM fraction; 4, UC fraction. (C) Western blotting analysis of rPnxIIIA by using anti-rPnxIIIA IgG after cross-linking with only rPnxIIIA or the rPnxIIIE protein and IP with anti-rPnxIIIA IgG. Lanes: 1, rPnxIIIA without cross-linking; 2, 20 μg of rPnxIIIA alone cross-linked with 50 mM BS3 for 60 min and immunoprecipitated; 3, mixture of both rPnxIIIA and rPnxIIIE proteins without cross-linking; 4, 20 μg of both rPnxIIIA and rPnxIIIE proteins cross-linked with 50 mM BS3 for 60 min and immunoprecipitated. (D) Western blotting analysis of rPnxIIIA by using anti-rPnxIIIA IgG after different treatment times with rPnxIIIA alone cross-linked with 50 mM BS3 and immunoprecipitated with anti-rPnxIIIA IgG. Lanes: 1, rPnxIIIA without cross-linking; 2, rPnxIIIA with cross-linking for 5 min; 3, rPnxIIIA with cross-linking for 10 min; 4, rPnxIIIA with cross-linking for 30 min; 5, rPnxIIIA with cross-linking for 60 min. Arrows indicate the position of the bands that appeared.

Sasaki et al. BMC Microbiology 2011 11:55   doi:10.1186/1471-2180-11-55
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