Table 3

Hemolysis, DNA fragmentation, cytotoxicity, and metabolic activity of Campylobacter concisus isolatesa.

Isolate

AFLP

Cluster

Hemolysisb

(%)

DNA fragmentationc

(A370 nm)

Cytotoxicityc

(%)

Metabolic activity c

(% control)


CHRB2004

1

60.2 ± 14.4

1.84 ± 0.17d

1.23 ± 0.21

139.4 ± 7.4

CHRB3287

1

45.6 ± 16.9

1.83 ± 0.13d

1.48 ± 0.16

146.8 ± 9.2

CHRB2011

1

60.5 ± 9.8

1.63 ±.0.05d

0.88 ± 0.22

151.9 ± 7.5

CHRB3290

1

81.1 ± 4.5

1.91 ± 0.14d

0.94 ± 0.19

155.7 ± 2.3

CHRB1609

1

72.3 ± 9.4

1.37 ± 0.18

1.11 ± 0.34

144.5 ± 4.4

CHRB1794

2

70.9 ± 10.1

1.32 ± 0.19

1.42 ± 0.15

137.9 ± 2.9

CHRB6

2

41.2 ± 11.6

1.12 ± 0.26

1.43 ± 0.18

105.1 ± 26.2e

CHRB1569

2

47.0 ± 12.0

1.38 ± 0.17

1.29 ± 0.26

139.2 ± 7.0

CHRB2691

2

62.1 ± 14.3

1.62 ± 0.07d

1.89 ± 0.15

133.5 ± 10.3

CHRB2370

2

44.9 ± 12.0

1.69 ± 0.14d

1.46 ± 0.08

142.8 ± 6.5

CHRB2050

2

64.3 ± 15.4

1.41 ± 0.07

0.97 ± 0.15

131.0 ± 7.1

CHRB563

2

34.6 ± 13.9

1.55 ± 0.23d

1.25 ± 0.20

138.0 ± 10.2

CHRB3152

2

30.7 ± 15.4

1.89 ± 0.16d

1.28 ± 0.15

141.0 ± 6.0

CHRB3235

2

32.1 ± 18.6

1.69 ± 0.12d

1.14 ± 0.16

143.2 ± 6.3

LMG7788

1

61.5 ± 10.8

1.54 ± 0.08d

0.71 ± 0.10

140.8 ± 5.2

C. jejuni 81-176

--

75.6 ± 3.7

1.68 ± 0.25d

4.53 ± 0.31d

143.7 ± 5.7

Broth control

--

0.44 ± 0.14

0.69 ± 0.12

0.96 ± 0.34

100

H2O2

--

--

1.38 ± 0.22

6.15 ± 1.66d

259.5 ± 13.5

Camptothecin

--

--

2.23 ± 0.40d

1.39 ± 0.28

177.5 ± 9.2


a Data are means ± SEM, n = 3.

b Percent total hemolysis of sheep erythrocytes for 1/8 dilution of Campylobacter inoculum.

c Assays conducted using T84 monolayers

d P < 0.05 relative to the broth control treatment.

e Sloughing of epithelial cells noted in two of three repetitions.

Kalischuk and Inglis BMC Microbiology 2011 11:53   doi:10.1186/1471-2180-11-53

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