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Characterization of N-acylhomoserine lactone-degrading bacteria associated with the Zingiber officinale (ginger) rhizosphere: Co-existence of quorum quenching and quorum sensing in Acinetobacter and Burkholderia

Kok-Gan Chan125*, Steve Atkinson2, Kalai Mathee3, Choon-Kook Sam4, Siri Ram Chhabra2, Miguel Cámara2, Chong-Lek Koh4 and Paul Williams2

Author Affiliations

1 Division of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, 50603 Kuala Lumpur, Malaysia

2 School of Molecular Medical Sciences, Centre for Biomolecular Sciences, University of Nottingham, Nottingham, NG7 2RD, UK

3 Department of Molecular Microbiology and Infectious Diseases, Herbert Wertheim College of Medicine, Florida International University, Miami, FL 33199, USA

4 Natural Sciences and Science Education AG, National Institute of Education, Nanyang Technological University, 1 Nanyang Walk, Singapore 637616, Singapore

5 Institute of Biological Sciences (Division of Genetics and Molecular Biology), Faculty of Science, University of Malaya, 50603 Malaysia

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BMC Microbiology 2011, 11:51  doi:10.1186/1471-2180-11-51

Published: 8 March 2011

Additional files

Additional file 1:

Mass spectra AHLs produced by GG4. Extracts from spent culture supernatants of GG4 were analysed by mass spectrometry. The peak ion at m/z 102 is characteristic of the homoserine lactone ring (A, B, E and F). By comparison with the corresponding synthetic standards (C, D, G and H) the precursor ion at m/z 214.2 and fragment ion at m/z 113.0 suggest the presence of 3-oxo-C6-HSL (A); the precursor ion at m/z 228.2 and fragment ion m/z 109.1 are indicative of C8-HSL (B); the precursor ion at m/z 226.2 [M-H2O] and fragment ion m/z 125.1 are indicative of 3-hydroxy-C8-HSL (E); the precursor ion at m/z 242.2 and fragment ion of m/z 142.2 are indicative of C9-HSL (F). AU: Absorbance unit.

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