sRNAs mapped to host target RNAs are enriched or depleted during DENV2 infection and show an inverse relationship to transcript levels. A) Enriched sRNAs categorized by target functional group in DENV2-infected samples over un-infected blood-fed controls. B) Depleted sRNAs categorized by target functional group in DENV2-infected samples over controls. C) Enriched sRNAs at 2 dpi categorized by sRNA size group. Targets of unknown function are not shown. D) Depleted sRNAs at 2 dpi categorized by sRNA size group. Targets of unknown function are not shown. 'ncRNA', non-coding RNAs, 'CSR', chemo-sensory receptor, 'TRP', transport (signal transduction, ion transport, transmembrane transport), 'PRO', protease, 'ReDox', oxidative reductive components not associated with the mitochondria, 'TT', Transcription/Translation mRNAs, 'MIT' mitochondrial function, 'LIPID_MET' Lipid_Metabolism, 'MET', general metabolism, 'IMM', immunity, 'DIV', diverse function, 'CYT/STR', cytoskeletal/structural. E) Selected target mRNAs were subjected to qRT-PCR analysis in pooled midguts. Bars represent percent change in 2 dpi DENV-2 infected RexD Ae. aegypti midguts versus un-infected control midguts from the same time-point. The Delta-delta Ct analytical method was applied and ribosomal protein S7 was used as reference standard. Target transcripts not maintaining the expected inverse relationship with sRNA profiles are marked with an asterisk.
Hess et al. BMC Microbiology 2011 11:45 doi:10.1186/1471-2180-11-45