Table 1

Plasmids used in this study

Plasmid

Relevant characteristic(s)*

Reference or source


pACYC184

cat, tetA, p15A ori

[59]

pBAD-HisA

amp, pBR ori

Invitrogen

pKD46

λ Red recombinase expression plasmid

[60]

p15A-PB2-kan

cat, kan, p15A ori

This study

pYA4463

pACYC184, adjacent 5'tet and 3'tet

This study

pYA4464

pACYC184, 3'tet

This study

pYA4465

pBAD-HisA; 5'tet

This study

pYA4590

pACYC184, 5'tet-kan-3'tet

This study

pYA4373

cat-sacB

[54]

pRE112

oriT, oriV, sacB, cat

[61]

pYA3886

pRE112, ΔrecF126

This study

pYA4783

pYA3886, ΔrecF1074

This study

pYA3887

pRE112, ΔrecJ1315

This study

pYA4680

pRE112, ΔrecA62

This study

pYA4518

pYA4464, cat, p15A ori, GFP gene

This study

pYA4518-cysG

Two cysG fragments

This study

pYA4689

pYA4518-cysG, 5'tet-kan-3'tet

This study

pYA4690

pYA4518-cysG, 5'tet-kan

This study

pYA5001

aacC1, pSC101 ori, T vector

This study

pYA5002

pYA5001, recA cassette from Typhimurium χ3761

This study

pYA5004

pYA5001, recA cassette from Typhi Ty2 χ3769

This study

pYA5005

pYA5001, recF gene from Typhimurium χ3761

This study

pYA5006

pYA5001, recF gene from Typhi Ty2 χ3769

This study


* cat: chloramphenicol resistance gene; tetA: tetracycline resistance gene; amp: ampicillin resistance gene; kan: kanamycin resistance gene; 3'tet: 3' portion of the tetA gene; 5'tet: 5' portion of the tetA gene together with its promoter; aacC1: 3-N-aminoglycoside acetyltransferase.

Zhang et al. BMC Microbiology 2011 11:31   doi:10.1186/1471-2180-11-31

Open Data