Open Access Research article

Genesis of a novel Shigella flexneri serotype by sequential infection of serotype-converting bacteriophages SfX and SfI

Qiangzheng Sun1, Ruiting Lan2, Yiting Wang1, Jianping Wang1, Xia Luo1, Shaomin Zhang1, Peijing Li1, Yan Wang1, Changyun Ye1, Huaiqi Jing1 and Jianguo Xu1*

Author Affiliations

1 State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, China CDC, P.O. Box 5, Changping, Beijing, China

2 School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW 2052, Australia

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BMC Microbiology 2011, 11:269  doi:10.1186/1471-2180-11-269

Published: 30 December 2011

Additional files

Additional file 1:

Supplementary figure. DNA sequences of integration sites in 036, 036_X and 036_1d, and bacteriophages SfI and SfX. Sequences obtained by PCR and sequencing of junction regions using a series of primers across the integration site as described in the text. (A) attB in strain 036. (B) attP in phage SfI. (C) attP in phage SfX. (D) attL in strain 036_X. (E) attR in 036_X and 036_1d. (F) Sequence between phage SfI and SfX in strain 036_1d. Sequences in box are conserved DNA regions between genes; Underlined sequences are tRNA-thrW; Sequences in blue are att core sequence; Conserved genes flanking a given integration site are shaded and their transcription orientation is marked by an arrow.

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