Table 1

Effect of high copy plasmid clones on survival following accumulation of mutant topoisomerase I cleavage complex

Plasmid

Survival Ratio


pCRII vector

7.85 × 10-5 ± 1.19 × 10-5


pAQ5

4.95 × 10-3 ± 1.55 × 10-3


pAQ5-1

4.92 × 10-3 ± 1.20 × 10-3


pAQ5-2

1.25 × 10-2 ± 2.48 × 10-3


pInter

1.90 × 10-2 ± 4.12 × 10-3


pInterD1

4.22 × 10-3 ± 1.02 × 10-3


pInterD2

5.19 × 10-4 ± 1.73 × 10-4


E. coli BW27784 carrying pAYTOP128 was transformed with high copy number plasmid shown in the table. Cultures were grown to exponential phase with shaking, then treated with 0.002% arabinose for 2.5 h before serial dilution and plating on LB plates with antibiotics and 2% glucose

Survival ratio was determined by calculating the ratio of the viable colony counts obtained from the induced cultures versus the viable counts from non-induced culture. The results represent the average and standard errors from at least three experiments

Liu et al. BMC Microbiology 2011 11:261   doi:10.1186/1471-2180-11-261

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