Addition of adenine to minimal medium increases survival following induction of mutant topoisomerase I cleavage complex BW27784 transformed with pAYTOP128 was grown overnight in RM minimal medium with 2% glucose to suppress mutant topoisomerase I expression, then diluted 1:100 into RM medium with 0.2% glycerol. When OD600 reached 0.4, 0.00008% or 0.0002% arabinose was added with or without 100 μg/ml adenine included. Viable colony counts were determined at 1 h and 2 h after arabinose addition (a). The presence of adenine did not affect expression of mutant YpTOP after induction of 0.0002% arabinose for 2 h as analyzed by Western blot (b).
Liu et al. BMC Microbiology 2011 11:261 doi:10.1186/1471-2180-11-261