Table 2

Plasmids used in this study

Plasmids

Genotype or description

Source or reference


pTSC1

A 6996-bp plasmid of strain X4-3

This work

pTSC2

A 7.5-kb plasmid of strain X3-3

This work

pTSC3

A 50-kb plasmid of strain T6-1-4

This work

pTSL1

A 16-kb linear plasmid of strain T6-1-4

This work

pSP72

amp colEI-ori

Life Technologies, Inc

pBluescript II SK

amp colEI-ori lacZ

Stratagene, Inc

pQC156

A 2.6-kb BclI-fragment of melC/tsr cloned in pSP72 (BglII)

[46]

pCWH1

A 7-kb KpnI fragment of pTSC1 cloned in pQC156

This work

pCWH100

A 7-kb KpnI fragment of pTSC1 cloned in pBluescript II SK

This work

pIJ702

melC tsr pIJ101 origin

[31]

pZR10

A 8.9-kb Sau3A1-fragment of pFP11 origin cloned in pQC156

[33]

pZR115

A 4.1-kb Sau3A1-fragment of pFP1 origin cloned in pQC156

[33]

pZR205

Two fragments (PCR) of SLP1 rep/imp cloned in pQC156

[33]

pZR51

A 2.2-kb HindIII fragment of pFRL2 origin cloned into pQC156

[32]

pHAQ61

A 2.9-kb fragment of SAP1 origin cloned in pQC156

Zhang and Qin, unpublished data

pYQ40

A 2-kb fragment of SCP2 origin cloned in pQC156

Yang and Qin, unpublished data

pGP9

A 4.1-kb EcoRI/BglII fragment of pSHK1 origin cloned in pQC156

[32]

pSET152

Streptomyces phage φC31-derived integration vector, aprr

[38]

pHAQ31

amp colEI-ori cos melC tsr

[47]

Cosmid N7-85

pHAQ31 (BamHI) containing c. 33 kb sequence (5510413-5543521 bp) from S. coelicolor A3(2)

This work

pCWH74

A 2.6-kb XbaI/NheI fragment containing the phiC31 integrase gene cloned in a pHAQ31-derived cosmid containing the actinorhodin biosynthetic gene cluster

This work

024CAO-3

The anthramycin biosynthetic gene cluster cloned into a cosmid CAO2

[22]


Chen and Qin BMC Microbiology 2011 11:243   doi:10.1186/1471-2180-11-243

Open Data