Figure 4.

Quantitation of actinorhodin production by M145 and by 4F containing the cloned actinorhodin gene cluster in liquid medium. About 1 × 106 spores of M145 and of 4F containing pCWH74 were inoculated into 50 ml R2YE liquid medium (lacking KH2PO4 and CaCl2) at 30 and 37°C. Samples of 1 ml culture were harvested in a time-course and treated with KOH; absorption at OD640 indicated actinorhodin production.

Chen and Qin BMC Microbiology 2011 11:243   doi:10.1186/1471-2180-11-243
Download authors' original image