Table 1

Genotypic and phenotypic characterization of S. aureus clinical isolates.

QRDR mutationsb

MIC (mg/L)c


EtBr

CIP

NOR

NAL


Isolatea

PFGE

pattern

GrlA

GyrA

No

+

+

No

+

+

No

+

+

No

+

+

EI

TZ

CPZ

EI

TZ

CPZ

EI

TZ

CPZ

EI

TZ

CPZ


ATCC25923

-

WT

WT

6.25

0.75

0.75

0.25

0.125

0.125

0.5

0.125

0.125

64

n.d.

n.d.

ATCC25923EtBr

-

WT

WT

200

25

12.5

1

0.25

0.25

2

0.25

0.25

64

n.d.

n.d.

SM1

A2

S80Y/E84K

S84L

16

4

4

128

32

64

512

128

256

256

64

64

SM10

A4

S80Y/E84K

S84L

16

2

4

128

64

64

512

128

128

128

64

64

SM14

A3

S80Y/E84K

S84L

16

4

4

256

32

128

1024

128

256

256

64

64

SM17

A4

S80Y/E84K

S84L

16

4

4

256

64

64

1024

256

512

256

64

64

SM25

A1

S80Y/E84K

S84L

8

2

4

128

32

64

512

64

128

256

32

64

SM27

A4

S80Y/E84K

S84L

16

4

4

256

32

64

512

128

256

256

64

64

SM43

A1

S80Y/E84K

S84L

16

2

4

128

64

64

512

128

128

512

256

64

SM46

A1

S80Y/E84K

S84L

16

4

4

128

64

64

512

128

256

128

64

64

SM47

A1

S80Y/E84K

S84L

8

2

4

256

32

64

512

128

256

256

64

64

SM48

A1

S80Y/E84K

S84L

8

4

4

256

32

64

512

128

256

256

64

64

SM50

B1

S80F/E84K

S84L

8

1

2

64

16

16

256

32

64

128

64

64

SM52

C1

E84K

S84L

16

1

2

16

8

8

64

32

32

128

32

64

SM2

B2

S80F/E84K

S84L

8

2

2

32

16

16

128

32

32

64

16

64

SM3

E1

S80Y/E84K

S84L

1

1

1

16

8

8

64

32

32

64

16

16

SM4

E2

S80F

S84L

4

2

1

8

8

8

64

32

32

64

32

64

SM5

E3

S80Y/E84K

S84L

4

2

1

32

16

16

128

64

64

64

32

32

SM6

A5

S80F

E88K

4

2

1

16

16

16

64

32

32

64

32

32

SM7

E1

S80F

S84L

2

2

1

8

8

4

64

32

32

128

32

64

SM8

A5

S80F

E88K

4

2

1

16

8

16

128

64

64

128

32

64

SM12

E1

S80F

S84L

2

2

1

16

8

8

64

32

32

128

32

64

SM16

A6

S80F

E88K

4

2

1

16

16

16

128

32

64

64

32

64

SM22

A1

S80Y/E84G

S84L

8

4

4

128

16

32

512

128

128

64

32

64

SM34

D1

S80F/E84K

S84L

4

2

2

64

16

32

64

16

32

32

16

32

SM36

E1

S80F

S84L

4

2

2

16

8

8

64

16

32

128

32

64

SM40

E1

S80F

S84L

8

4

4

32

32

32

512

128

128

16

8

16


aIsolates in bold correspond to the EtBrCW-positive isolates. bWT: wild-type; S: serine; F: phenylalanine; E: glutamate; K: lysine; Y: tyrosine; L: leucine; G: glycine. cValues in bold-type correspond to a MIC decrease of ≥ four-fold in the presence of the efflux inhibitor (EI) in comparison to the values with no EI [10]. The concentration of each EI used is defined in the Methods section. EtBr: ethidium bromide; CIP: ciprofloxacin; NOR: norfloxacin; NAL: nalidixic acid; TZ: thioridazine; CPZ: chlorpromazine; n.d.: not determined.

Costa et al. BMC Microbiology 2011 11:241   doi:10.1186/1471-2180-11-241

Open Data