ALN has a differential ability to bind to erythrocyte cell membranes from different host species. His-ALN (500 ng) or buffer (negative control) was added to erythrocytes, and the mixture was incubated on ice for 20 min. Untreated (no reactivity, data not shown) or ALN-treated erythrocyte membrane fractions from human (H), bovine (B), ovine (O) or rabbit (R) blood were separated by SDS-PAGE, transferred to nitrocellulose, and immunostained with 1/1000 rabbit anti-His-ALN. His-Aln (500 ng) in absence of erythrocyte membrane fractions (ALN) serves as the positive control. Molecular mass markers (kDa) are indicated on the left.
Jost et al. BMC Microbiology 2011 11:239 doi:10.1186/1471-2180-11-239