Quantification of the transcripts of the core regions of ICESt1 (A) and ICESt3 (B). Arrows correspond to transcripts. Primer pairs used for cDNA quantification are represented by convergent triangles below the corresponding transcript. Other symbols used in the map are identical to those used in Figure 1. cDNA quantities determined from cells grown in LM17 medium and harvested in exponential growth phase (expo0.6) or stationary phase (stat) or after 2.5 hours of exponential growth with mitomycin C (MMC) at MIC/2 are normalized to the quantity of cDNA of gyrA whose transcription is constitutive . Lack of amplicon is mentioned as non-detected (ND). For each condition, data are average and standard deviation from three independent biological replicates.
Carraro et al. BMC Microbiology 2011 11:238 doi:10.1186/1471-2180-11-238