Table 1

Bacterial strains and plasmids used in this study

Strains and plasmids

Genotype/phenotype/source

Origin or reference


Streptococcus thermophilus


LMG18311

Wild-type; isolated from yogurt.

BCCM LMG

Δrgg0182

Derivative of LMG18311 carrying a 699 bp deletion in the rgg0182 gene

This study

LMG18311 blp::Ppep0182-luxAB

LMG18311 containing a Ppep0182-luxAB fusion at the blp locus

This study

LMG18311 blp::Pshp0182-luxAB

LMG18311 containing a Pshp0182-luxAB fusion at the blp locus

This study

Δrgg0182 blp::Ppep0182-luxAB

Δrgg0182 containing a Ppep0182-luxAB fusion at the blp locus

This study

Δrgg0182 blp::Pshp0182-luxAB

Δrgg0182 containing a Pshp0182-luxAB fusion at the blp locus

This study

Escherichia coli


EC101

supE hsd-5 thi Δ(lac-proAB) F'(traD6 proAB+ lacIq lacZΔM15) repA+, derivative of TG1 strain

[37,38]

EC101 pG9Δrgg0182

Derivative of EC101 containing pG9Δrgg0182

This study

C41(DE3)

Derived from BL21(DE3) (F- ompT hsdSB(rB- mB-) gal dcm met (DE3); Novagen); at least one uncharacterized mutation

[39]

C41(DE3) pET15b::rgg0182

Derivative of C41(DE3) containing pET15b::rgg0182

This study

Plasmid


pG+host9

Thermosensitive plasmid replication origin from pVE6002, used for gene replacement; EryR

[36]

pG9Δrgg0182

Derivative of pG+host9 carrying a 699 bp (from nucleotide 82 to 783) deletion for rgg0182

This study

pGICB004

Thermosensitive plasmid, Emr; used for the construct of transcriptional fusions. Allow the integration of the luxAB genes of Photorabdus luminescens at the blp locus of the S. thermophilus chromosome

[9]

pGICB004::Ppep0182

Derivative of pGICB004 used to introduce a Ppep0182-luxAB transcriptional fusion at the blp locus

This study

pGICB004::Pshp0182

Derivative of pGICB004 used to introduce a Pshp0182-luxAB transcriptional fusion at the blp locus

This study

pET15b

Expression vector for N-terminal His6-tagged fusion; AmpR

Novagen

pET15b::rgg0182

Derivative of pET15b carrying a 864 bp insert coding a Rgg0182 His6-tagged protein

This study


BCCM Belgian Coordinated Collections of Microorganisms, LMG Laboratory of Microbiology and Genetics, University of Gent

Henry et al. BMC Microbiology 2011 11:223   doi:10.1186/1471-2180-11-223

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