Analysis of the Rgg0182 binding to DNA. Electrophoretic mobility shift assay (EMSA) of the promoter regions of the two target genes (shp0182 and pep0182) of Rgg0182 in the absence or in the presence of the purified His6-Rgg0182 protein. DNA probes labelled with biotin (0.1 pmol each) were incubated with 2 pmol of Rgg0182. The Pldh probe is an ldh promoter fragment used as a negative control.
Henry et al. BMC Microbiology 2011 11:223 doi:10.1186/1471-2180-11-223