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Open Access Highly Accessed Research article

Comparison of PCR ribotyping and multilocus variable-number tandem-repeat analysis (MLVA) for improved detection of Clostridium difficile

Hsiao L Wei12, Chun Wei Kao1, Sung H Wei3, Jason TC Tzen2* and Chien S Chiou1*

Author Affiliations

1 The Central Region Laboratory, Center for Research and Diagnostics, Centers for Disease Control, 5F 20 Wen-Sin South 3rd Road, Taichung City 40855, Taiwan

2 Graduate Institute of Biotechnology, National Chung-Hsing University, 250 Guoguang Road, Taichung City 40277, Taiwan

3 The Third Branch Office, Centers for Disease Control, Taiwan

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BMC Microbiology 2011, 11:217  doi:10.1186/1471-2180-11-217

Published: 30 September 2011

Additional files

Additional file 1:

Copy numbers, fragment sizes, sequences, and GenBank accession number of each allele at 40 VNTR loci. This table provides the copy number and fragment sizes of the six initially test strains. The copy numbers (or array sizes) in each allele, their corresponding sequence, and their GenBank accession number are shown.

Format: XLS Size: 190KB Download file

This file can be viewed with: Microsoft Excel Viewer

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Additional file 2:

Allelic number and allele of VNTR loci in each PCR ribotype. This table provides the allelic number and allele of VNTR loci in each PCR ribotype, and only allelic number larger than one are listed.

Format: XLS Size: 24KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 3:

Epidemiological data, toxigenic type, and molecular type of isolates from one hospital in central Taiwan. This table provides the molecular typing data of MLVA10 and MLVA4 for C. difficile isolates from one hospital in Taiwan, and the corresponding epidemiological data and characteristic of each strain are shown.

Format: XLS Size: 28KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data

Additional file 4:

Allelic diversity of MLVAs in each PCR ribotype. This table provides the Simpson's allelic diversity of either types or groups from MLVA10 and MLVA34 panels.

Format: XLS Size: 16KB Download file

This file can be viewed with: Microsoft Excel Viewer

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Additional file 5:

Primers for amplification of each locus. This table provides a list of primers, annealing temperature, and primer concentration for amplification of each VNTR loci.

Format: XLS Size: 29KB Download file

This file can be viewed with: Microsoft Excel Viewer

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Additional file 6:

List of predictable VNTR loci at 75%, 70%, and 65% predictable value. This table provides the list of VNTR loci which could be predicted by loci in MLVA12, MLVA10, and MLVA8.

Format: XLS Size: 24KB Download file

This file can be viewed with: Microsoft Excel Viewer

Open Data