Novel transcripts are validated by RT-PCR. RT-PCR products for primer pairs targeting TYR1 (positive control) and 22 novel transcripts detected on the whole genome tiling arrays. A standard DNA ladder flanks each gel. "RT" indicates whether reverse transcriptase was added to the cDNA synthesis reaction.
Voorhies et al. BMC Microbiology 2011 11:216 doi:10.1186/1471-2180-11-216