Table 3

Vectors and interaction summary

Vector pair(s)

Fusions proteins

Interactions*


pDEST22/pDEST32

N/N (N-terminal fusions)

8

pGADT7g/pGBKT7g

N/N (N-terminal fusions)

44

pGBKT7g/pGADCg

N/C (N-terminal/C-terminal fusions)

39

pGBKCg/pGADCg

C/C (C-terminal/C-terminal fusions)

18

pGBKCg/pGADT7g

C/N (C-terminal/N-terminal fusions)

26


* Redundant, i.e. some interactions are found with multiple vectors.

Fusion proteins indicate the location of the DNA-binding (DBD) and activation domains (AD), respectively, of each vector pair. For instance, the pDEST vectors both have the DBD and AD fused at the N-terminus of the bait and prey protein. Vectors are listed as bait/prey pairs.

Rajagopala et al. BMC Microbiology 2011 11:213   doi:10.1186/1471-2180-11-213

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