Table 3

The effect of 20 mM external CaCl2 (in Vogels* medium) on the growth inhibitory activity of AFPNN5353 on A. niger strain A533.

AFPNN5353 (μg/ml)

Vogels*

Vogels* + 20 mM Ca2+


0

100 (SD ± 10)

100 (SD ± 8)

0.5

12 (SD ± 3)

101 (SD ± 9)

1.0

no growth

105 (SD ± 6)


OD620 was measured after 24 h of incubation. The growth of untreated controls was normalized to 100% to evaluate the percent growth of samples in the presence of AFPNN5353. Vogels* medium without CaCl2 supplementation contains 0.7 mM Ca2+. Results are expressed as mean ± SD (n = 3).

Binder et al. BMC Microbiology 2011 11:209   doi:10.1186/1471-2180-11-209

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