Indirect immunofluorescence staining of A. nidulans with rabbit anti-AFPNN5353 antibody. Fungi were incubated with 0.2 μg/ml AFPNN5353 (A, E, G) or without antifungal protein (C). 20 μg/ml latrunculin B (E) and 10 mM Ca2+ (G) significantly reduced protein uptake. (B, D, F, H) are the respective light microscopic images of (A, C, E, G). Scale bar 10 μm.
Binder et al. BMC Microbiology 2011 11:209 doi:10.1186/1471-2180-11-209