Global population structure of Aspergillus terreus inferred by ISSR typing reveals geographical subclustering
1 Mycotic Diseases Branch, Center for Disease Control and Prevention, 1600 Clifton Rd NE, Atlanta, GA, 30329, USA
2 Department of Plant Biology, The University of Georgia at Athens, 2502 Miller Plant Sciences, Athens, GA 30602, USA
3 Dipartimento di Sanità Publica-Microbiologia-Virologia, Università degli Studi di Milano via Pascal 36, 20133 Milano, Italy
4 Division of Infectious Diseases, Department of Medicine, Santa Clara Valley Medical Center, Stanford University Medical School, 751 So. Bascom Av., San Jose, CA 95128, USA
BMC Microbiology 2011, 11:203 doi:10.1186/1471-2180-11-203Published: 16 September 2011
Aspergillus terreus causes invasive aspergillosis (IA) in immunocompromised individuals and can be the leading cause of IA in certain medical centers. We examined a large isolate collection (n = 117) for the presence of cryptic A. terreus species and employed a genome scanning method, Inter-Simple Sequence Repeat (ISSR) PCR to determine A. terreus population structure.
Comparative sequence analyses of the calmodulin locus revealed the presence of the recently recognized species A. alabamensis (n = 4) in this collection. Maximum parsimony, Neighbor joining, and Bayesian clustering of the ISSR data from the 113 sequence-confirmed A. terreus isolates demonstrated that one clade was composed exclusively of isolates from Europe and another clade was enriched for isolates from the US.
This study provides evidence of a population structure linked to geographical origin in A. terreus.