Electrophoretic profiles of semi-purified Cetavlon precipitates and biofilm. Bacteria were grown anaerobically on plates or to late stationary phase, Cetavlon added, and precipitates extracted, as described in Methods. Each extract was loaded onto 25% polyacrylamide gels, followed by electrophoresis and staining with Alcian blue and silver. Lanes: 1 and 2, 20 μg and 30 μg of EPS extracted under growth conditions favorable to biofilm formation; 3 and 4, 20 μg and 30 μg of EPS extracted from cells grown to late stationary phase in broth, respectively; 5, buffer alone; 6 and 7, 20 μg and 30 μg of EPS extracted from cells grown anaerobically on plates, respectively.
Sandal et al. BMC Microbiology 2011 11:186 doi:10.1186/1471-2180-11-186