Determination of TLRs (+) cells in histological sections of small intestine. The samples were obtained before the infection for the untreated control (C) and healthy mice given L. casei CRL431 (Lc group), and 7 and 10 days post challenge for all experimental groups. The number of fluorescent cells was counted in 30 fields of vision at 1 000X of magnification and the results were expressed as the number of positive cells counted per 10 fields. The microphotographs (400×) F and H show the increases of TLR2+ and TLR4+ cells, respectively (fluorescent cells) in mice from Lc group compared to the untreated control (C group: E for TLR2 and G for TLR4). Means for each value without a common letter differ significantly (P < 0.01).
Castillo et al. BMC Microbiology 2011 11:177 doi:10.1186/1471-2180-11-177