Figure 3.

SDS-PAGE of outer membrane protein preparations stained with Coomassie Blue. OM proteins were extracted from 24-hour-old populations of bacteria grown on solid minimal medium with 0.2% glucose. Representative results of the P. putida PaW85 (wt), colR-deficient (colR), and of different transposon insertion derivatives of the colR-deficient strains are shown. Arrows indicate locations of the channel proteins OprB1 and OprF (calculated molecular weights 49.6 kD and 37 kD, respectively). All lanes contain 0.5 μg of OM proteins.