Representative examples of ICC flow cytometry analysis of PBMC in response to p1L and rPPE44. The percentage of IFN-γ+ CD4+ cells is given in the upper right corner of each panel. Panel A, PBMC from a PPD- healthy donor in the presence of p1L; panel B and C, PBMC of a PPD+ healthy donor in the presence of p1L and rPPE44, respectively.
Cuccu et al. BMC Microbiology 2011 11:167 doi:10.1186/1471-2180-11-167