Co-Immunoprecipitation and Western Blot of SSCMK1 and HSP90. This figure shows the results obtained with co-immunoprecipitation and Western Blot analysis of SSCMK1 interacting with SSHSP90.Whole cell free extracts of S. cerevisiae cells expressing the complete c-myc tagged SSCMK1coding sequence fused to the GAL4 activation domain (bait protein) and the HA tagged protein fragment fused to the GAL4 DNA binding domain (prey protein) were co-immunoprecipitated as described in Methods. The co-immunoprecipitated proteins were separated using 10% SDS polyacrylamide electrophoresis and transferred to nitrocellulose. The nitrocellulose strips were probed with anti-cMyc antibodies (Lane 1) and anti HA antibodies (Lane 3). Pre-stained molecular weight markers were included in outside lanes of the gel. The position of the molecular weight markers is indicated in the figure. Lanes 2 and 4 are negative controls where no primary antibody was added.
Rodriguez-Caban et al. BMC Microbiology 2011 11:162 doi:10.1186/1471-2180-11-162