Reactivity of recombinant MBP-fusion proteins containing epitopes TATTEK (MBP-Cp-2) and VVDGPETKEC (MBP-Dp-1) with WNV/JEV-positive equine serum by WB. MBP alone or MBP fused with the TATTEK (MBP-Cp-2) and VVDGPETKEC (MBP-Dp-1) peptides were evaluated by WB for reactivity with antibodies in WNV/JEV-positive equine serum. MBP-fused proteins containing the two epitopes reacted with WNV-positive equine serum (Fig. 6 a, b) and WNV-negative equine serum (Fig. 6 c, d). The polypeptide Dp-1 and Cp-2 reacted with six JEV-positive equine sera, respectively (Fig. 6 e and f). M: PageRuler™ Prestained Protein Ladder (Fermentas, Canada).
Sun et al. BMC Microbiology 2011 11:160 doi:10.1186/1471-2180-11-160