Figure 6.

Reactivity of recombinant MBP-fusion proteins containing epitopes TATTEK (MBP-Cp-2) and VVDGPETKEC (MBP-Dp-1) with WNV/JEV-positive equine serum by WB. MBP alone or MBP fused with the TATTEK (MBP-Cp-2) and VVDGPETKEC (MBP-Dp-1) peptides were evaluated by WB for reactivity with antibodies in WNV/JEV-positive equine serum. MBP-fused proteins containing the two epitopes reacted with WNV-positive equine serum (Fig. 6 a, b) and WNV-negative equine serum (Fig. 6 c, d). The polypeptide Dp-1 and Cp-2 reacted with six JEV-positive equine sera, respectively (Fig. 6 e and f). M: PageRulerâ„¢ Prestained Protein Ladder (Fermentas, Canada).

Sun et al. BMC Microbiology 2011 11:160   doi:10.1186/1471-2180-11-160
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