a) Plasmid profiles of CFNX101 (lane 1) and CFNX101/pDOP-CsA (lane 2), showing that plasmid p42d and pDOP-CsA are compatible. b) Linear representation of constructs containing SymA repC gene (blue arrow), p42d repC gene (red arrow) and SymA/p42d hybrid derivatives (blue/red arrows), and their associated replication capabilities when introduced into R. etli CFNX101 (with p42d) and CFNX107 (a p42d cured derivative) strains (table at left). "+" Symbols indicate that the construct are capable to replicate, and "-" that the construct is incapable to do that. Construct names are listed at the right of the figure. Black squares indicate the relative position of the Plac promoter, and the white rectangles the position of the Shine-Dalgarno (SD) sequences. Numbers at top indicate the positions where the SymA/p42d regions were swap.
Cervantes-Rivera et al. BMC Microbiology 2011 11:158 doi:10.1186/1471-2180-11-158